Commonly this pressure is 0.25 inches of drinking water column, but can be raised or decreased as the method requires. Isolators have to be periodically leak tested to make certain their integrity and forestall escape from the decontamination agent. Leak testing can be done by various methods such as https://tailinscitech.wixsite.com/tailinscitech/post/applications-and-benefits-of-tailin-containment-isolator-for-handling-high-potency-materials